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Protein Phosphatase Protocols

Author : Greg Moorhead
Publisher : Springer Science & Business Media
Release Date : 2008-02-05
ISBN 10 : 9781597452670
Pages : 387 pages
Rating : 4.6/5 (452 downloads)

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Download Protein Phosphatase Protocols PDF Format Full Free by Greg Moorhead and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Phosphatase Protocols presents a broad range of protocols for the study of protein phosphatases, all written by experts and innovators from phosphatase laboratories around the world. This volume is a compendium of resources for the study of protein phosphatases and their potential as drug targets. Experimental methodologies are taken from proteomics, bioinformatics, genomics, biochemistry, RNAi, and genetics.

Plant Molecular Biology Manual

Author : Stanton Gelvin
Publisher : Springer Science & Business Media
Release Date : 2012-12-06
ISBN 10 : 9789400909519
Pages : 492 pages
Rating : 4.5/5 (99 downloads)

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Download Plant Molecular Biology Manual PDF Format Full Free by Stanton Gelvin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past ten years, great advances have been made in the area of plant molecular biology. Such formerly esoteric techniques as gene transfer and plant regeneration are now routinely performed, making the dissection of regulatory elements of genes a common practice in many laboratories. Along with this new technology has come an almost bewildering array of rapidly changing techniques, often making it difficult for the novice to select and perform the technique most appropriate for answering a given biological question. In 1986, some of us felt that many of these techniques had become routine enough to warrant the publication of a laboratory manual. The manual is designed both for advanced college level laboratory courses and as a 'bench guide' for use in the scientific laboratory. Recognizing the rapidly changing nature of plant molecular biology technology, the editors have designed a laboratory manual that is both easy to use in the laboratory and which will be updated as the techniques change and new technologies are devised. Additional chapters that can replace or be added to this first edition will be published periodically. The editors recognize that many of the techniques described in this manual depend upon specialized plant genetic material, microbial strains, or recombinant plasmids. Those people desiring such material should contact the relevant authors directly. A list of the various contributors to this manual, including their addresses, is included.

Quantum Puzzle, The: Critique Of Quantum Theory And Electrodynamics

Author : Clarke Barry R
Publisher : World Scientific
Release Date : 2017-04-27
ISBN 10 : 9789814696999
Pages : 400 pages
Rating : 4.9/5 (696 downloads)

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Download Quantum Puzzle, The: Critique Of Quantum Theory And Electrodynamics PDF Format Full Free by Clarke Barry R and published by World Scientific. This book was released on 2017-04-27 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1861, James Clerk–Maxwell published Part II of his four-part series "On physical lines of force". In it, he attempted to construct a vortex model of the magnetic field but after much effort neither he, nor other late nineteenth century physicists who followed him, managed to produce a workable theory. What survived from these attempts were Maxwell's four equations of electrodynamics together with the Lorentz force law, formulae that made no attempt to describe an underlying reality but stood only as a mathematical description of the observed phenomena. When the quantum of action was introduced by Planck in 1900 the difficulties that had faced Maxwell's generation were still unresolved. Since then theories of increasing mathematical complexity have been constructed to attempt to bring the totality of phenomena into order with little success. This work examines the problems that had been abandoned long before quantum mechanics was formulated in 1925 and argues that these issues need to be revisited before real progress in the quantum theory of the electromagnetic field can be made. Contents:IntroductionThe Faraday–Maxwell FieldsThe ElectronBlackbody RadiationAtomic StructureLight and ActionMass Vortex RingsThe Magnetic Vortex FieldThe Electric Vortex Field Readership: Advanced undergraduate and graduate students interested in quantum physics.

Nonradioactive Analysis of Biomolecules

Author : Christoph Kessler
Publisher : Springer Science & Business Media
Release Date : 2012-12-06
ISBN 10 : 9783642572067
Pages : 750 pages
Rating : 4.0/5 (572 downloads)

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Download Nonradioactive Analysis of Biomolecules PDF Format Full Free by Christoph Kessler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 750 pages. Available in PDF, EPUB and Kindle. Book excerpt: New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids. The second edition of this successful laboratory manual describes in detail the highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation as well as PCR amplification. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques, enabling scientists to plan, design and conduct the appropriate procedures.

Single Molecule Toroics

Author : Keith Murray
Publisher : Springer Nature
Release Date : 2022-12-25
ISBN 10 : 9783031117091
Pages : 242 pages
Rating : 4.0/5 (117 downloads)

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Download Single Molecule Toroics PDF Format Full Free by Keith Murray and published by Springer Nature. This book was released on 2022-12-25 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book consists of chapters written by international experts on various aspects of single molecule toroics (SMTs).The chapters cover a broad range of relevant topics and highlight the latest advances performed in the field. An up-to-date overview of the emerging SMT architectures is presented while particular attention is given to not only the magnetism and relaxation effects involved but also to the respective applications in advanced electronics and memory devices. The role that lanthanides play -especially that of dysprosium- is discussed, while a thorough analysis using theoretical/ab initio calculations is provided. Since SMTs have grown out of single molecule magnetism (SMM), it is an expanding and topical subject and the present book will engender excitement and interest amongst chemists, physicists, theoreticians and materials scientists. The volume will be of great interest to researchers and graduates working on this topic and particularly those involved in lanthanide chemistry, magnetism and theory.

C. elegans

Author : Kevin Strange
Publisher : Springer Science & Business Media
Release Date : 2008-02-05
ISBN 10 : 9781597451512
Pages : 292 pages
Rating : 4.5/5 (451 downloads)

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Download C. elegans PDF Format Full Free by Kevin Strange and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular biology has driven a powerful reductionist, or “molecule-c- tric,” approach to biological research in the last half of the 20th century. Red- tionism is the attempt to explain complex phenomena by defining the functional properties of the individual components of the system. Bloom (1) has referred to the post-genome sequencing era as the end of “naïve reductionism. ” Red- tionist methods will continue to be an essential element of all biological research efforts, but “naïve reductionism,” the belief that reductionism alone can lead to a complete understanding of living organisms, is not tenable. Organisms are clearly much more than the sum of their parts, and the behavior of complex physiological processes cannot be understood simply by knowing how the parts work in isolation. Systems biology has emerged in the wake of genome sequencing as the s- cessor to reductionism (2–5). The “systems” of systems biology are defined over a wide span of complexity ranging from two macromolecules that interact to carry out a specific task to whole organisms. Systems biology is integrative and seeks to understand and predict the behavior or “emergent” properties of complex, multicomponent biological processes. A systems-level characteri- tion of a biological process addresses the following three main questions: (1) What are the parts of the system (i. e.

Novel Superfluids

Author : Karl-Heinz Bennemann
Publisher : Oxford University Press
Release Date : 2013-02-28
ISBN 10 : 9780199585915
Pages : 641 pages
Rating : 4.9/5 (585 downloads)

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Download Novel Superfluids PDF Format Full Free by Karl-Heinz Bennemann and published by Oxford University Press. This book was released on 2013-02-28 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reports on the latest developments in the field of Superfluidity, one of the most fundamental, interesting, and important problems in physics, with applications ranging from metals, helium liquids, photons in cavities, excitons in semiconductors, to the interior of neutron stars and the present state of the Universe as a whole.

Microarrays

Author : Jang B. Rampal
Publisher : Springer Science & Business Media
Release Date : 2007-07-27
ISBN 10 : 9781588295897
Pages : 453 pages
Rating : 4.8/5 (295 downloads)

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Download Microarrays PDF Format Full Free by Jang B. Rampal and published by Springer Science & Business Media. This book was released on 2007-07-27 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments. Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology. Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.

Plant Proteostasis

Author : L. Maria Lois
Publisher : Springer Nature
Release Date : 2022-11-22
ISBN 10 : 9781071627846
Pages : 408 pages
Rating : 4.8/5 (627 downloads)

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Download Plant Proteostasis PDF Format Full Free by L. Maria Lois and published by Springer Nature. This book was released on 2022-11-22 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field.

New and Emerging Proteomic Techniques

Author : Dobrin Nedelkov
Publisher : Springer Science & Business Media
Release Date : 2008-02-04
ISBN 10 : 9781597450263
Pages : 248 pages
Rating : 4.2/5 (45 downloads)

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Download New and Emerging Proteomic Techniques PDF Format Full Free by Dobrin Nedelkov and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Transcription Factors

Author : David Latchman
Publisher : OUP Oxford
Release Date : 1999-04-08
ISBN 10 : 9780191565793
Pages : 325 pages
Rating : 4.7/5 (565 downloads)

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Download Transcription Factors PDF Format Full Free by David Latchman and published by OUP Oxford. This book was released on 1999-04-08 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the first edition five years ago, a wide range of new methodologies have been developed to facilitate studies on both isolated parts of the genome and the genome as a whole. This new edition has been updated and expanded so that it provides a comprehensive guide to the methods currently available to characterize the function and activity of an individual transcription factor. All the original chapters have been fully updated or rewritten and additional chapters cover the use of in vitro transcription assays, analysis of chromatin structure, use of the genomic binding site assay and analysis of transcription factor modifications. As with the previous edition, the book starts with a series of chapters concerned with characterizing the proteins binding to a specific DNA sequence and then a chapter on more detailed characterization of the protein itself. The next two chapters describe the isolation of cDNA clones encoding a transcription factor using oligonucleotides predicted from protein sequence and screening of a cDNA expression library. Chapter 6 deals with identification of transcription factors based on sequence homology analysis by both experimental screening and database searches. Chapter 7 is a new chapter that describes methods of identifying the target genes of a previously uncharacterized factor. The next chapters deal with analysis of transcription factor function. Chapter 8 deals with general techniques, and then the following chapters cover the specialized techniques of in vitro transcription assays using transcriptionally active nuclear extracts derived from rat brain, and analysis of the effect of transcription factors on chromatin structure. The final chapter describes methods for detecting the phosphorylation and glycosylation state of transcription factors.

Adoptive Immunotherapy

Author : Burkhard Ludewig
Publisher : Springer Science & Business Media
Release Date : 2008-02-02
ISBN 10 : 9781592598625
Pages : 500 pages
Rating : 4.6/5 (598 downloads)

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Download Adoptive Immunotherapy PDF Format Full Free by Burkhard Ludewig and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immunotherapy, and the utilization of regulatory T cells. The protocols make it possible to study the adoptive transfer of tailored antigen-specific immune cells and to improve the clinical application of adoptive immunotherapy.

Topological Defects and the Non-Equilibrium Dynamics of Symmetry Breaking Phase Transitions

Author : Yuriy M. Bunkov
Publisher : Springer Science & Business Media
Release Date : 2012-12-06
ISBN 10 : 9789401141062
Pages : 394 pages
Rating : 4.0/5 (141 downloads)

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Download Topological Defects and the Non-Equilibrium Dynamics of Symmetry Breaking Phase Transitions PDF Format Full Free by Yuriy M. Bunkov and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: Topological defects formed at symmetry-breaking phase transitions play an important role in many different fields of physics. They appear in many condensed-matter systems at low temperature; examples include vortices in superfluid helium-4, a rich variety of defects in helium-3, quantized mag netic flux tubes in type-II superconductors, and disclination lines and other defects in liquid crystals. In cosmology, unified gauge theories of particle interactions suggest a sequence of phase transitions in the very early uni verse some of which may lead to defect formation. In astrophysics, defects play an important role in the dynamics of neutron stars. In 1997 the European Science Foundation started the scientific network "Topological defects" headed by Tom Kibble. This network has provided us with a unique opportunity of establishing a collaboration between the representatives of these very different branches of modern physics. The NATO-ASI (Advanced Study Institute), held in Les Houches in February 1999 thanks to the support of the Scientific Division of NATO, the European Science Foundation and the CNRS, represents a key event of this ESF network. It brought together participants from widely different fields, with diverse expertise and vocabulary, fostering the exchange of ideas. The lectures given by particle physicists, cosmologists and condensed matter physicists are the result of the fruitful collaborations established since 1997 between groups in several European countries and in the U.S.A.

RNA Turnover in Bacteria, Archaea and Organelles

Author : Lynne E. Maquat
Publisher : Academic Press
Release Date : 2008-12-23
ISBN 10 : 0080922066
Pages : 620 pages
Rating : 4.9/5 (8 downloads)

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Download RNA Turnover in Bacteria, Archaea and Organelles PDF Format Full Free by Lynne E. Maquat and published by Academic Press. This book was released on 2008-12-23 with total page 620 pages. Available in PDF, EPUB and Kindle. Book excerpt: Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. Covers the difference in processing of mRNA between eukaryotes, bacteria and archea. Benefit: Processing of mRNA differs greatly between eukaryotes, bacteria and archea and this affords researchers readily reproducible techniques to understand and study the molecular pathogenesis of disease Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover which are central to the process of gene expression. Benefit: Keeps MIE buyers and online subscribers up-to-date with the latest research Offers step by step lab instructions including necessary equipment and reagents. Benefit: Provides tried and tested techniques which eliminate searching through many different sources. Tested techniques are trustworthy and avoid pitfalls so the same mistakes are not made over and over

RNA Turnover in Eukaryotes: Nucleases, Pathways and Analysis of mRNA Decay

Author : Lynne E. Maquat
Publisher : Academic Press
Release Date : 2009-01-30
ISBN 10 : 0080922074
Pages : 656 pages
Rating : 4.9/5 (8 downloads)

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Download RNA Turnover in Eukaryotes: Nucleases, Pathways and Analysis of mRNA Decay PDF Format Full Free by Lynne E. Maquat and published by Academic Press. This book was released on 2009-01-30 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover, which are central to the process of gene expression Offers step-by-step lab instructions, including necessary equipment and reagents

Coherent States

Author : D H Feng
Publisher : World Scientific
Release Date : 1994-05-26
ISBN 10 : 9789814602518
Pages : 600 pages
Rating : 4.5/5 (62 downloads)

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Download Coherent States PDF Format Full Free by D H Feng and published by World Scientific. This book was released on 1994-05-26 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book consists of lectures delivered at the International Symposium on Coherent States: Past, Present, and Future, held in Oak Ridge, Tennessee, June 14 – 17 1993. Both theoretical and experimental subjects are treated. Theoretical subjects dealt with include quantum optics, quantum chaos, condensed matter physics, nuclear physics, high energy physics and foundational issues such as quantum-classical connections and various semiclassical quantization schemes. Experimental topics dealt with principally concern atomic and molecular physics and especially lasers. Topics related to coherent states, most notably wavelets, are also included. Contents: Quantum Versus Classical Phase in Optical Systems (G S Agarwal)Coherent States: How Far Can One Go? (J-P Antoine)Orthonormal Bases of Coherent States: The Canonical Case and the axGroup (I Daubechies)Dynamics of Coherent States as the Diffusion Process: Schrödinger's System, Bernstein Processes, and Quantum Evolution (P Garbaczewski)Some Reflections on Coherence and Ion Trapping (R J Glauber)Coherent States, Chaos, and Information (E J Heller & S Tomsovic)Coherent States as a Tool for Obtaining Rigorous Bounds (E H Lieb)Classical-Quantum Correspondence in Time-Dependent Perturbation Theory (S M McRae & E R Vrscay)Asymptotic Coherence of Gluons and of q-Bosons (C A Nelson)Electron Nuclear Dynamics with Coherent States (Y Öhrn & E Deumens)Quantum Coherent Operators (J P Solovej)Classicalization and Coherent States (W-M Zhang & D H Feng)Post-Banquet Talk (A S Wightman)and other papers Readership: Theoretical and applied physicists and mathematicians.

Chemosensitivity

Author : Rosalyn D. Blumenthal
Publisher : Springer Science & Business Media
Release Date : 2005
ISBN 10 : 9781592598892
Pages : 452 pages
Rating : 4.8/5 (598 downloads)

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Download Chemosensitivity PDF Format Full Free by Rosalyn D. Blumenthal and published by Springer Science & Business Media. This book was released on 2005 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemosensitivity testing is an ex vivo means of determining or enhancing the cytotoxic and/or cytostatic, or apoptosis-inducing effects of anticancer drugs. In Chemosensitivity, leading researchers and physicians working in academia and biotech companies describe their best laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Volume 2: In Vivo Models, Imaging, and Molecular Regulators contains today's best protocols for classifying tumors into response categories and for customizing therapy to individuals. These techniques allow measurements of DNA damage, apoptotic cell death, and the molecular and cellular regulators of cytotoxicity, as well as in vivo animal modeling of chemosensitivity. Highlights include genomic and proteomic approaches to assess chemosensivity, in vivo imaging approaches to assess early response to therapy, and methods to statistically analyze data from in vivo therapy. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The authors also provide guidance on how best to analyze the data derived from the protocols. A companion volume, Volume 1: In Vitro Assays contains in vitro and in vivo techniques to identify which new agents or combination of agents are effective for each type of tumor. Cutting-edge and highly practical, the two volumes of Chemosensitivity provide a comprehensive collection of readily reproducible techniques for the in vitro and in vivo screening of new agents and a set of proven approaches to understand mechanistically why certain cancer cell lines (in vitro) of tumors (in vivo) are more or less sensitive to a particular agent.